Human Umbilical Cord Mesenchymal Stromal Cell Isolation, Expansion, Cryopreservation, and Characterization. (16th May 2017)

• Record Type: Journal Article
• Authors: Smith, J. Robert; Cromer, Adrienne; Weiss, Mark L.
• Editors: Bhatia, Mick; Elefanty, Andrew G.; Fisher, Susan J.; Patient, Roger; Schlaeger, Thorsten; Snyder, Evan Y.
• Is Part Of: Current protocols in stem cell biology. Volume 41(2018)
• Page Start: 1F.18.1
• Page End: 1F.18.23
• Publication Date: 2017-05-16
• Subjects: mesenchymal stromal cells (MSCs) -- umbilical cord (UC) -- stem cells -- isolation -- cryopreservation -- culture -- characterization -- platelet lysate; Stem cells -- Laboratory manuals; Stem cells -- Physiology; Stem Cells; Stem cells; Laboratory Manual; Laboratory manuals; 571.5
• DOI: 10.1002/cpsc.24
• Languages: English
• ISSNs: 1941-7322

Abstract

Abstract: Revised methods to derive, expand, and characterize mesenchymal stromal cells (MSCs) from the umbilical cord are provided. Several considerations are taken for GMP compliance including using a closed system isolation method and eliminating several xenogenic components. With this method cells are isolated using mechanical and enzymatic digestion and then expanded with high viabilities that retain >90% viability after cryopreservation. Lastly, characterization methods have been optimized to identify these cells as MSCs according to the ISCT minimal criteria. This method standardizes the process for isolating, expanding, cryopreserving, and characterizing MSCs from the umbilical cord. © 2017 by John Wiley & Sons, Inc.