Cytocompatible and non-fouling zwitterionic hyaluronic acid-based hydrogels using thiol-ene "click" chemistry for cell encapsulation. (15th May 2020)

• Record Type: Journal Article
• Authors: Zhang, Yabin; Liu, Shuyan; Li, Tianyu; Zhang, Luqing; Azhar, Umair; Ma, Jiachen; Zhai, Congcong; Zong, Chuanyong; Zhang, Shuxiang
• Is Part Of: Carbohydrate polymers. Volume 236(2020)
• Publication Date: 2020-05-15
• Subjects: Hydrogel -- "Thiol-ene" click chemistry -- Protein resistance -- 3D cell encapsulation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116021
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: A facile click reaction strategy is employed to form hydrogels in situ . The clickable hydrogel based on modified hyaluronic acid and zwitterionic polymer. The hydrogels can be applied for 3D cell encapsulation. Abstract: In this work, a facile click reaction strategy is employed to form hydrogels in situ with cytocompatibility, biodegradability, self-healing property and resistance to protein. The thiol-functionalized zwitterionic carboxybetaine methacrylate copolymer, which take part as a cross-linker in the "thiol-ene" click reaction with the methacrylated hyaluronic acid. The hydrogels are obtained under the physiological condition without the presence of any copper catalyst and UV light. The hydrogel consisting of zwitterionic component shows an obvious reduction in protein adsorption and cell adhesion and avoid non-targeted factor interference in the biological experiments. The hydrogels also demonstrate adjustable degradation behavior. Human mesenchymal stem cells (hMSCs) are easily encapsulated into the hydrogels and remains metabolically active, indicating the excellent biocompatibility of the hydrogels. Additionally, the result of the cytokine secretion assays (IL-6 and TNF-α) has shown that this clickable hydrogel can serve to suppress inflammatory reactions and is beneficial for in vivo applications. Based on the above results, this clickable hydrogel with excellent performance can be an amenable platform for 3D cell encapsulation.