Unravelling the antifungal and anti-aflatoxin B1 mechanism of chitosan nanocomposite incorporated with Foeniculum vulgare essential oil. (15th May 2020)

• Record Type: Journal Article
• Authors: Kumar, Akshay; Pratap Singh, Prem; Prakash, Bhanu
• Is Part Of: Carbohydrate polymers. Volume 236(2020)
• Publication Date: 2020-05-15
• Subjects: Antifungal -- Chitosan nanocomposite -- Eessential oil -- Foeniculum vulgare -- Mode of action; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116050
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Chemical characterization of Foeniculum vulgare essential oil (FvEO). FvEO was loaded inside the chitosan nanocomposite (Ne-FvEO). Ne-FvEO enhances the antifungal and aflatoxin B1 inhibitory activity. Antifungal and aflatoxin B1 inhibitory mode of action of Ne-FvEO. Ne-FvEO preserve the sensory and nutritional properties of Sorghum bicolor. Abstract: The study reports the antifungal and aflatoxin B1 inhibitory efficacy of chemically characterized chitosan-based nanoencapsulated Foeniculum vulgare Mill. essential oil (Ne-FvEO), and its effect on sensory and nutritional properties of Sorghum bicolor . The Ne-FvEO was characterized through SEM, FTIR and XRD. The Ne-FvEO demonstrated superior antifungal (0.4 μl/ml) and aflatoxin B1 inhibitory (0.3 μl/ml) performance than the free FvEO. The biochemical studies reveal the significant alteration in ergosterol content, ions leakage, mitochondrial membrane potential, C-sources utilization and antioxidant defense system in A. flavus exposed to Ne-FvEO. The 3D modeling of the Nor-1 gene product was done using the I-TASSER server and validated by the Ramachandran plot. The in-situ result reveals that the Ne-FvEO significantly preserved the nutritional and sensory characteristics of S. bicolor seeds. Therefore, Ne-FvEO could be used as a green preservative agent to enhance the shelf-life of the food commodities.