Physiological and antioxidant responses of Euryale ferox salisb seedlings to microcystins. (30th January 2021)

• Record Type: Journal Article
• Authors: Qian, Zong-Yao; Guo, Ya-Xin; Yin, Yu-Lai; Sun, Fang-Fang; Gong, Ting-Ting; Xian, Qi-Ming
• Is Part Of: Toxicon. Volume 190(2021)
• Page Start: 50
• Page End: 57
• Publication Date: 2021-01-30
• Subjects: Microcystin-LR -- Oxidative stress -- Antioxidant defense stress -- Euryale ferox Salisb seedlings; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2020.12.008
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Lake Taihu is the third largest freshwater lake located in eastern China. In recent years, it has experienced extensive cyanobacterial (Microcystis spp.) blooms that produce toxic microcystins (MCs), which may have acute and chronic hepatotoxic effects in animals and humans. Although the impact of MCs on both terrestrial and aquatic plants is well documented, the effects and underlying mechanisms of the harmful toxin MC-LR on Euryale ferox Salisb seedlings have rarely been reported. Thus, herein, the antioxidant response mechanisms and the biosynthesis of secondary metabolites during the exposure of E. ferox Salisb seedlings to varying MC-LR concentrations (0.05, 0.2, 1, and 5 μg/L) were thoroughly investigated after exposure periods (7, 14, 21 d). Our study revealed that the seedling growth was inhibited with increasing MC-LR exposure concentration that significantly induced at 1 μg/L and reached a maximum level at 5 μg/L, whereas the activity of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) in the seedling cells increased gradually with increasing MC-LR concentration and longer exposure time. The maximum malondialdehyde (MDA) content was 4.3-fold higher than that of the control group under an MC-LR concentration of 5.0 μg/L after 7 days of exposure treatment. The study of the seedling detoxification mechanism revealed that the content of total glutathione (tGSH) and reduced glutathione (GSH), as well as the activities of GSH sparse transferase (GST) and glutathione reductase (GR), increased to varying degrees and reached a maximum level at 1 μg/L. Therefore, the exposure to MC-LR can promote the accumulation of secondary metabolites and increase the activities of secondary metabolic enzymes in the seedlings. Further investigation of these antioxidative mechanisms will provide additional information for the identification and development of bio-indicators to evaluate the environmental impact of MCs on aquatic ecosystems. Graphical abstract: Image 1 Highlights: Microcystin-LR exposure can inhibit the growth of seedlings and cause serious oxidative stress. Microcystin-LR exposure enhanced the antioxidant enzymes activities of seedlings. Secondary metabolic-associated signaling are key events in detoxification process.