Reduced PCR cycling time amplification using AmpFℓSTR® Identifiler® kit. (December 2015)

• Record Type: Journal Article
• Authors: Iyavoo, S.; Wolejko, A.; Furmanczyk, D.; Graham, R.; Myers, R.; Haizel, T.
• Is Part Of: Forensic science international. Volume 5(2015)
• Page Start: e286
• Page End: e288
• Publication Date: 2015-12
• Subjects: DNA -- PCR -- STR -- Reduced time -- Identifiler; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2015.09.113
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: To reduce the DNA profiling time, DNA samples were amplified using AmpFℓSTR ® Identifiler ® kit at reduced PCR cycling time amplification method without addition of any enhancement buffers or enzymes. The results showed no significant difference in the quality of the profiles produced compared to those produced in the manufacturer recommended condition. This study contributed in saving almost 25% of the time needed for PCR amplification without compromising the quality of the profiles.