Drimys winteri and isodrimeninol decreased foam cell formation in THP-1 derived macrophages. (December 2020)

• Record Type: Journal Article
• Authors: Burgos, Viviana; Paz, Cristian; Saavedra, Kathleen; Saavedra, Nicolás; Foglio, Mary Ann; González-Chavarría, Iván; Salazar, Luis A.
• Is Part Of: Food and chemical toxicology. Volume 146(2020)
• Publication Date: 2020-12
• Subjects: Drimys winteri -- Isodrimeninol -- Foam cell -- THP-1 -- PMA; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2020.111842
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Early stages of atherosclerosis are characterizated for the uptake of oxidate low-density lipoprotein (oxLDL) by inflammatory macrophages in the arteries, promoting the foam cell formation. Drimys winteri is a native tree of Chile that produce drimane sesquiterpenoids, here it was evaluated the inhibitory foam cell formation by the total extract of barks of Drimys winteri and isodrimeninol, a sesquiterpenoid isolated from the tree. The results showed that Dw and isodrimeninol inhibited the foam cell formation on macrophage M1, by Oil Red O staining. Moreover, Dw reduced the gene expression of pro-inflammatory cytokine TNF-α, in contrast to isodrimeninol that showed not effect on the gene expression of this cytokine, also Dw enhanced the expression of the anti-inflammatory cytokine IL-10, in more significant manner than isodrimeninol at 20 μg/mL. While, Dw and isodrimeninol significantly reduced the expression of IL1-β at concentrations of 20 μg/mL, but not affecting the MMP-9 levels, assessed by RT-qPCR. In conclusion, Drimys winteri and isodrimeninol induce anti-atherosclerotic effects, inhibiting foam cell formation, as well as promoting anti-inflammatory responses. This study confirm the relevance of this tree as a medicinal source for the Mapuche people, and suggesting that Drimys winteri could be used in early stages of atherosclerosis. Highlights: Drimys winteri produces isodrimeninol, a bioactive hemiacetal drimane sesquiterpenoid. Isodrimeninol significatively decreases the foam cell formation and IL1β. Isodrimeninol increases the anti-inflammatory IL10 at 20 μg/mL. Total extract of Drimys winteri decreases the inflammatory signals TNFα and IL1β, as well as the foam cell formation.