Application of molecularly imprinted polymers and dual-emission carbon dots hybrid for ratiometric determination of chloramphenicol in milk. (December 2020)

• Record Type: Journal Article
• Authors: Jalili, Roghayeh; Khataee, Alireza
• Is Part Of: Food and chemical toxicology. Volume 146(2020)
• Publication Date: 2020-12
• Subjects: Ratiometry -- Chloramphenicol -- Carbon dots -- Nanosensor -- Point of care testing; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2020.111806
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Chloramphenicol (CLP) is a veterinary antibiotic that has been banned due to its severe side effects but it is still illegally used in animal husbandry. In this work, the fabrication of simple, fast-response and highly selective ratiometric probe for sensitive visual detection of CLP antibiotic at trace levels in both indoor and outdoor is reported. For the construction of the ratiometric fluorescence probe (mMIP@YBCDs), two kinds of different carbon dots with yellow emission (Y/CDs, 560 nm) and blue emission carbon dots (B/CDs, 440 nm) were used as target sensitive and as reference dyes respectively. Besides, molecularly imprinted mesoporous silica was used as a recognized part of the probe. Upon the addition of different concentrations of CLP, the fluorescence of Y/CDs was quenched significantly while the fluorescence intensity of B/CDs stayed constant which was accompanied by gradual fluorescence color change from yellow-to-blue. The ratiometric probe has a linear response in the range of 0.1–3 μgL −1 with a detection limit 0.035 μgL −1 . The practicality of the ratiometric method was validated by the quantification of CLP in milk samples. Graphical abstract: Image 1 Highlights: Green source carbon dots with different emission colors were synthesized. A novel molecular imprinting ratiometric fluorescence sensor was developed. The detection limit of the probe can achieve 0.035 μgL −1 with high selectively. Ratiometric fluorescence technique was adopted to achieve visual signal output.