Structure of a laminarin-type β-(1→3)-glucan from brown algae Sargassum henslowianum and its potential on regulating gut microbiota. (1st March 2021)

• Record Type: Journal Article
• Authors: Cui, Yongsheng; Zhu, Lixia; Li, Yixuan; Jiang, Siliang; Sun, Qili; Xie, Enyi; Chen, Hubiao; Zhao, Zhongzhen; Qiao, Wei; Xu, Jun; Dong, Caixia
• Is Part Of: Carbohydrate polymers. Volume 255(2021)
• Publication Date: 2021-03-01
• Subjects: Sargassum henslowianum -- β-(1→3)-Glucan -- In vitro fermentation -- Gut microbiota -- Short-chain fatty acids; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.117389
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A laminarin-type glucan named SHNP was first isolated from Sargassum henslowianum . SHNP contains a β -D-(1→ 3)-glucan backbone with single t - β -D-Glc p as side chains branched at O-6 position. SHNP promotes the production of SCFAs and regulates the composition of gut microbiota. SHNP has the potential for regulating gut microbiota. Abstract: A homogeneous polysaccharide named SHNP with apparent molecular weight of 8.4 kDa was purified from brown algae Sargassum henslowianum using ethanol precipitation, ion-exchange chromatography, and gel-filtration column chromatography. Structural analyses reveal that SHNP is completely composed of glucose, and its backbone consists of β -D-(1→ 3)-Glc p with side chains comprising t - β -D-Glc p attached at the O-6 position. Thus, SHNP is a laminarin-type polysaccharide. In vitro fermentation test results showed that SHNP was digested by gut microbiota; the pH value in the fecal culture of SHNP was significantly decreased; and total short-chain fatty acids, acetic, propionic and n-butyric acids were significantly increased. Furthermore, SHNP regulated the intestinal microbiota composition by stimulating the growth of species belonging to Enterobacteriaceae while depleting Haemophilus parainfluenzae and Gemmiger formicilis . Taken together, these results indicate that SHNP has the potential for regulating gut microbiota, but its specific role in the regulation requires to be further investigated.