Artemisia biennis Willd.: Anti-Nociceptive effects and possible mechanisms of action. (25th March 2021)

• Record Type: Journal Article
• Authors: Zarei, Mohammad; Ahmadimoghaddam, Davoud; Mohammadi, Saeed
• Is Part Of: Journal of ethnopharmacology. Volume 268(2021)
• Publication Date: 2021-03-25
• Subjects: Artemisia biennis -- Camphor -- Opioid receptor -- L arginine-NO-cGMP-K(ATP) -- Peripheral antinociception; Naltrindole -- PubChem CID: 5497186 -- Nor-binaltorphimine -- PubChem CID: 5480230 -- Naloxonazine -- PubChem CID: 9576413 -- Methylene blue -- PubChem CID: 6099 -- Glibenclamide -- PubChem CID: 3488 -- L-arginine -- PubChem CID: 6322 -- Nitroprusside sodium -- PubChem CID: 11953895 -- Glutamate -- PubChem CID: 33032 -- Morphine -- PubChem CID: 5288826 -- Naloxone -- PubChem CID: 5464092 -- Diazepam -- PubChem CID: 6452650 -- Nω-nitro-L-arginine methyl Ester hydrochloride (L-NAME) -- PubChem CID:39836; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2020.113604
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance : Artemisia biennis Willd. (Dermane in Persian) has been used as an antinociceptive remedy in Iranian folkloric medicine. Objective: The aim of the present study was to evaluate the anti-nociceptive effects of Artemisia biennis Willd. aerial part essential oil (ABAEO) on male Swiss mice. Materials and methods: Nociceptive pain techniques including acetic acid-induced writhing (AAIW), formalin-induced paw licking (FPL), glutamate-induced paw licking (GPL), and tail-flick (TF) models were applied. We assessed opioid and L-arginine-NO-cGMP-KATP pathways to detect the possible anti-nociceptive properties of ABAEO. In addition, neuropathic pain was induced by the cervical spinal cord contusion model. Results: ABAEO (120 mg/kg) had a significant anti-nociceptive activities in comparison to the control animals (p < 0.05) in the AAIW, TF, GPL, and FPL assays. The selective opioid antagonist (naloxonazine) administration in the AAIW test alleviated the anti-nociceptive effect of ABAEO (p < 0.05). L-arginine, methylene blue, and glibenclamide treatment prevented the ABAEO anti-nociceptive effects (p < 0.05); however, sodium nitroprusside could profoundly potentiate the ABAEO-associated antinociception in the FPL (phase II) test (p < 0.05). In nociceptive pain models, Cr (one of the main constituents of ABAEO) showed significant anti-nociceptive effects (p < 0.05). Moreover, the von Frey results indicated that ABAEO could attenuate mechanical allodynia in mice. Conclusion: Our observation revealed the anti-nociceptive effects of ABAEO in male mice. These effects could include, at least in part, modulating glutamatergic mechanisms via opioid systems. Our data output also indicates activating the L-arginine-NO-cGMP-KATP system in ABAEO anti-nociceptive activities. Graphical abstract: Image 1