Comparison of effects of high- and low-frequency electromagnetic fields on proliferation and differentiation of neural stem cells. (10th January 2021)

• Record Type: Journal Article
• Authors: Bai, Wenfang; Li, Meihui; Xu, Weicheng; Zhang, Mingsheng
• Is Part Of: Neuroscience letters. Volume 741(2021)
• Publication Date: 2021-01-10
• Subjects: Electromagnetic field -- Intensity -- Neural stem cell -- Proliferation -- Differentiation; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2020.135463
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Transcranial magnetic stimulation (TMS) has been widely applied to diagnose and treat intractable brain diseases. Both 50 Hz LF-EMF and HF-EMF can promote the proliferation of NSCs in vitro. LF-EMF can accelerate NSCs to differentiate into neurons. Abstract: To compare the effects of high- (HF-EMF) and low-frequency electromagnetic fields (LF-EMF) on the proliferation and differentiation of neural stem cells (NSCs). NSCs were obtained from SD rat hippocampus and cultured in suspension and adherent differentiation media. NSCs were exposed to LF-EMF (5 m T, 50 Hz, 30 min daily), HF-EMF (maximum magnetic induction 2.5 T, 40 % MO, 50 Hz, 10 min daily) and no electromagnetic field. At 3 d, cell viability and quantity of NSCs in suspension were detected by CCK-8 assay and cell counting plate. Immunofluorescence staining and qRT-PCR were performed to detect the percentage of Tu j -1 and GFAP-positive NSCs and the expression of Tuj-1 and GFAP mRNA. The P3 NSCs were positive with Nestin and induced NSCs expressed Tuj-1, GFAP and oligodendrocyte markers (MBP). CCK-8 assay and cell counting showed that the OD value and quantity of LF-EMF group were significantly higher than those in other two groups (both P < 0.05). Compared with the control group, the OD value and quantity were significantly higher in the HF-EMF group ( P < 0.05). Immunofluorescence staining and qRT-PCR revealed that the percentage of Tuj-1 positive cells and the expression of Tuj-1 mRNA of NSCs exposed to LF-EMF were the highest (both P < 0.05). The proportion of GFAP-positive NSCs and the expression of GFAP mRNA did not significantly differ among three groups (all P > 0.05). Both 50 Hz LF-EMF and HF-EMF can promote the proliferation of NSCs in vitro and LF-EMF can accelerate NSCs to differentiate into neurons.