Degenerated Primers for The KCS Enzyme Gene Encoding Erucic Acid Content in Winged Bean Seeds. Issue 1 (September 2020) - Sasongko, N D
Abstract: Winged bean (Psophocarpus tetragonolobus (L.) DC.) contains moderate to high fat in its seeds including erucic acid (C22:1) which is an important raw-material of various oleochemical industries. β-ketoacyl-CoA synthase (KCS) is one among those enzymes involves in erucic acid biosynthesis pathway by condensation acyl-CoA to β-ketoacyl-CoA. Information about encoding genes of the KCS enzyme involve in erucic acid biosynthesis pathway, therefore, it becomes a prerequisite. Current data show the paired degenerated primers of forward primer 5'-ATNTTCAACCCGACDCCDTC-3' and reverse 5'-GAGCTTCACCTCCAACATYS-3'. PCR resulted in amplicons of 404 bp and 301 bp which are predicted as putative fragments of the KCS enzyme encoding genes involved in the erucic acid biosynthesis in winged bean.