Akt Phosphorylation Orchestrates T11TS Mediated Cell Cycle Arrest in Glioma Cells. (26th November 2021)

• Record Type: Journal Article
• Authors: Acharya, Sagar; Chatterjee, Sirshendu; Chaudhuri, Suhnrita; Singh, Manoj Kumar; Bhattacharya, Debanjan; Bhattacharjee, Malabika; Ghosh, Anirban; Chaudhuri, Swapna
• Is Part Of: Cancer investigation. Volume 39:Number 10(2021)
• Page Start: 854
• Page End: 870
• Publication Date: 2021-11-26
• Subjects: Glioma -- T11TS -- Akt -- cell cycle arrest -- apoptosis; Cancer -- Periodicals; Oncology -- Periodicals; Medical Oncology -- Periodicals; Neoplasms -- Periodicals; 616.994
• DOI: 10.1080/07357907.2021.1986060
• Languages: English
• ISSNs: 0735-7907

Abstract

Abstract: The novel anti-neoplastic glycopeptide T11TS retards glioma both in in-vitro clinical samples and in-vivo models. This study investigates the correlation between altering the glioma microenvironment with glioma arrest and death. Flow cytometry, immunoblotting, ELISA, and co-immunoprecipitation were employed to investigate glioma cell arrest and death. Results include a decline in phosphorylation of Akt and attenuation of p21 phosphorylation (Thr145, Ser146) and disassociation of p-Akt-Mdm2 and p-Akt-BAD facilitating death by Akt>BAD. T11TS influence phosphorylation patterns in two focal axes Akt>p21 and Akt>Mdm2>p53. The current article provides crucial insight in deciphering the mechanism of T11TS induced glioma cell arrest and death.