Newcastle disease virus vectors expressing consensus sequence of the H7 HA protein protect broiler chickens and turkeys against highly pathogenic H7N8 virus. Issue 35 (14th August 2019)

• Record Type: Journal Article
• Authors: Roy Chowdhury, Ishita; Yeddula, Sai Goutham Reddy; Pierce, Brian G.; Samal, Siba K.; Kim, Shin-Hee
• Is Part Of: Vaccine. Volume 37:Issue 35(2019)
• Page Start: 4956
• Page End: 4962
• Publication Date: 2019-08-14
• Subjects: Highly pathogenic avian influenza virus -- Newcastle disease virus vectors -- Vaccines -- Prime-boost vaccination -- Chickens -- Turkeys; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2019.07.028
• Languages: English
• ISSNs: 0264-410X

Abstract

Highlights: Newcastle disease virus vectored H7 serotype-specific vaccines was generated. Heterologous prime and boost immunization was conducted. Vaccine candidates completely protected broiler chickens against H7N8 HPAI virus. Abstract: Continuous outbreaks of highly pathogenic avian influenza (HPAI) viruses in commercial poultry have caused devastating losses to domestic poultry with a raising public health concern. The outbreaks of HPAI viruses have increased worldwide, including the North America. Therefore, vaccination has been considered as an alternative strategy for an efficient control of HPAI viruses. In this study, we aimed to generate Newcastle disease virus (NDV) vectored H7 serotype-specific vaccines by expressing the consensus sequence of the HA protein. Conventional NDV strain LaSota vector and a chimeric NDV vector containing the avian paramyxovirus type-2 F and HN protein were able to express the consensus sequence of HA protein. The protective efficacy of vaccines was evaluated in broiler chickens and in turkeys. One-day-old poults were prime immunized with the chimeric vector expressing the HA protein followed by boost immunization with LaSota vector expressing the HA protein or co-expressing the HA and NA proteins. Our vaccine candidates provided complete protection of broiler chickens from mortality and shedding of H7N8 HPAI challenge virus. Turkeys were better protected by boosting with the LaSota vector co-expressing the HA and NA proteins than the LaSota vector expressing only the HA protein. Our study demonstrated a potential use of heterologous prime and boost vaccination strategy to protect poultry against H7 HPAI viruses.