Ameliorative effects of Osteo-F, a newly developed herbal formula, on osteoporosis via activation of bone formation. (25th March 2021)

• Record Type: Journal Article
• Authors: Lee, Haesu; Kim, Mi Hye; Choi, La Yoon; Yang, Woong Mo
• Is Part Of: Journal of ethnopharmacology. Volume 268(2021)
• Publication Date: 2021-03-25
• Subjects: Osteoporosis -- Osteo-F -- BMD -- Osteocalcin -- bALP -- BMP-2 -- OPN; ALP alkaline phosphatase -- bALP bone-specific ALP -- BMC bone mineral content -- BMD bone mineral density -- BMP-2 bone morphogenetic protein-2 -- COL pro-collagen type I -- DXA dual energy X-ray absorptiometry -- E2 17β-estradiol -- LV lumbar vertebrae -- OC osteocalcin -- OPN osteopontin -- Osx osterix -- OVX ovariectomy -- PTH human parathyroid hormone -- Runx2 runt related transcription factor 2; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2020.113590
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: The fruit of Schizandra chinensis and Lycium chinense, and the root of Eucommia ulmoides, components of Osteo-F, has traditionally been used for treating bone diseases in Korean Medicine. Aim of the study: The exact role and underlying mechanism of Osteo-F herbal formula on bone formation in osteoporosis was investigated in the present study. Materials and methods: OVX mice were treated with 0.9, 9 and 90 mg/kg of Osteo-F for 4 weeks. Bone tissues including fourth to sixth lumbar vertebrae (LV) and femur were collected to analyze the bone mineral density (BMD). In addition, serum biomarkers were estimated by enzyme-linked immunosorbent assay. The expressions of collagen, BMP-2 and osteopontin were determined in tibia to clarify the bone anabolic effects of Osteo-F in osteoporosis. Results: The levels of BMD in both of fourth to sixth LV and femur were significantly increased by Osteo-F treatment in OVX mice. Bone mineral content (BMC) was also elevated in Osteo-F-treated LV and femoral bone tissues. In addition, serum osteocalcin was markedly increased by Osteo-F in osteoporotic mice. Serum ALP and bALP levels were neutralized in Osteo-F 90 mg/kg-administered mice. Furthermore, Osteo-F treatment dramatically increased the mRNA expressions of collagen type I, BMP-2 and OPN in tibial bone specimens. Conclusions: Osteo-F ameliorated bone loss by increasing bone forming molecules including BMP-2 and OPN in osteoporosis. Osteo-F, a newly developed herbal formula, may be an alternative material for the management of osteoporosis with bone anabolic effects. Graphical abstract: Osteo-F consisting of the fruit of Schizandra chinensis and Lycium chinense, and the root of Eucommia ulmoides ameliorated bone loss in osteoporosis via bone forming BMP-2 signaling. Image 1 Highlights: Osteo-F ameliorated the loss of BMD and BMC in osteoporotic mice. Osteo-F recovered serum osteocalcin, ALP and bALP concentrations. Osteo-F increased levels of COL, OPN and BMP-2 in bone.