SiRNA knockdown of the DEK nuclear protein mRNA enhances apoptosis and chemosensitivity of canine transitional cell carcinoma cells. Issue 1 (April 2015)

• Record Type: Journal Article
• Authors: Yamazaki, Hiroki; Iwano, Tomomi; Otsuka, Saori; Kagawa, Yumiko; Hoshino, Yuki; Hosoya, Kenji; Okumura, Masahiro; Takagi, Satoshi
• Is Part Of: Veterinary journal. Volume 204:Issue 1(2015)
• Page Start: 60
• Page End: 65
• Publication Date: 2015-04
• Subjects: DEK nuclear protein -- Dog -- Transitional cell carcinoma -- SiRNA; Veterinary medicine -- Periodicals; 636
• DOI: 10.1016/j.tvjl.2015.02.009
• Languages: English
• ISSNs: 1090-0233

Abstract

Highlights: DEK protein was expressed at high levels in canine transitional cell carcinoma (TCC). Levels of DEK mRNA in four TCC cell lines were higher than those of other oncogenes. SiRNA knockdown of DEK enhanced apoptosis and chemosensitivity in four TCC cell lines. Abstract: Transitional cell carcinoma (TCC) in dogs is an aggressive malignant neoplasm, originating in the epithelium of the urinary bladder. The DEK nuclear protein is overexpressed in several types of human bladder cancer, where it is involved in chromatin reconstruction, gene transcription and apoptosis. Since DEK represents a potential therapeutic target for canine TCC, this study was designed to investigate DEK expression in canine TCC and to determine the effects of DEK mRNA silencing on TCC cells in vitro. The gene expression profiles of seven selected cancer-associated genes was assessed in four canine TCC cell lines and expression of DEK protein was evaluated in bladder tissue biopsies from healthy dogs and those affected with cystitis or TCC. After transfection of four canine TCC cell lines with DEK-specific or scrambled siRNA, annexin V staining was performed to evaluate apoptosis, and methylthiazole tetrazolium assays were performed to assess both cell viability and sensitivity to carboplatin. DEK mRNA expression was relatively high in canine TCC cells and expression of the DEK protein was significantly greater in TCC tumours compared with the other tissue samples. After transfection with DEK-specific siRNA, apoptosis, cell growth inhibition, and enhanced sensitivity to carboplatin were observed in all TCC cells assessed. These research findings suggest that DEK could be a potential therapeutic target for canine TCC.