Industrially relevant pectinase production from Aspergillus parvisclerotigenus KX928754 using apple pomace as the promising substrate. Issue 1 (1st January 2021)

• Record Type: Journal Article
• Authors: Satapathy, Sonali; Soren, Jyoti Prakash; Mondal, Keshab Chandra; Srivastava, Shweta; Pradhan, Chinmay; Sahoo, Santi Lata; Thatoi, Hrudayanath; Rout, Jyoti Ranjan
• Is Part Of: Journal of Taibah University for science. Volume 15:Issue 1(2021)
• Page Start: 347
• Page End: 356
• Publication Date: 2021-01-01
• Subjects: Agro-based residue -- apple pomace -- Aspergillus parvisclerotigenus -- pectinase -- liquid static surface fermentation; Science -- Periodicals; Science; Periodicals; 505
• DOI: 10.1080/16583655.2021.1978833
• Languages: English
• ISSNs: 1658-3655

Abstract

Abstract : The present study was focused on the potentiality of agro-based residues for the production of pectinase to meet the growing market demand by improving the yield with low cost of production. Among the agro-based residues used for the production of pectinase, apple pomace was able to produce the maximum of 1366.30 ± 36.71 U/ml using Aspergillus parvisclerotigenus KX928754 in liquid static surface fermentation, followed by sugarcane bagasse (973.12 ± 22.43 U/ml) and used tea (686.7 ± 45.06 U/ml). The process parameters optimization using a single variable at a time affirmed that pH 7.0, incubation period of 168 h, 30°C temperature, sucrose 2% as carbon source and peptone 3% as nitrogen source was found to be optimum for better production. The crude filtrate was purified by precipitation, dialyzed, eluted on Sephadex G-100 column followed by lyophilization and stored at −20°C. A. parvisclerotigenus KX928754 pectinase was purified to 2.10-fold, 2.91% of yield rate and having a specific activity of 1081.66 U/mg. Moreover, the electrophoretic analysis through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed 37.4 kDa of protein from the purified pectinase. Thus, the use of apple pomace as a substrate for scaling up pectinase with efficient recovery could reduce the price of the enzyme and increase its avenue for different industrial exploitation.