Biodegradation of aflatoxin B1 with cell-free extracts of Trametes versicolor and Bacillus subtilis. (December 2020)

• Record Type: Journal Article
• Authors: Suresh, Gayatri; Cabezudo, Ignacio; Pulicharla, Rama; Cuprys, Agnieszka; Rouissi, Tarek; Brar, Satinder Kaur
• Is Part Of: Research in veterinary science. Volume 133(2020)
• Page Start: 85
• Page End: 91
• Publication Date: 2020-12
• Subjects: Aflatoxin B1 -- Microbial degradation -- Cell-free extracts -- Degradation products -- Detoxification; AFB1 Aflatoxin B1 -- CFE Cell-free extracts -- HPLC High-Performance Liquid chromatography -- LC-MS/MS Liquid Chromatography with tandem mass spectrometry -- LAB Lactic acid bacteria -- ABTS 2, 2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid -- ACN Acetonitrile; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2020.09.009
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Aflatoxin B1 (AFB1 ) is one of the most common contaminants of poultry feed and has been linked to adverse effects on animal health and productivity. In this study, the degradation of AFB1 was studied with cell-free extracts (CFE) of Trametes versicolor and Bacillus subtilis using High-Performance Liquid chromatography (HPLC). CFE from B. subtilis and T. versicolor gave 60% and 34% of AFB1 degradation respectively, while heat-inactivated extracts showed no degradation. By-products obtained at the end of AFB1 degradation were analyzed by Liquid Chromatography with tandem mass spectrometry (LC-MS/MS). After 96 h of incubation, by-products with lower m / z values were obtained with CFE from B. subtilis as compared to that from T. versicolor, indicating a higher degradation efficiency of the former. Additionally, the detection of a by-product which could correspond to AFB1-8, 9 dihydrodiol - a less toxic derivative of AFB1 - after 72 and 96 h of incubation with CFE from B. subtilis, could indicate the simultaneous detoxification along with degradation of AFB1 by B. subtilis CFE. Graphical abstract: Unlabelled Image Highlights: AFB1 degradation with T. versicolor and B. subtilis cell-free extract (CFE). 60% and 34% AFB1 degradation with CFE of B. subtilis and T. versicolor respectively. No AFB1 degradation observed with heat-inactivated CFE. Degradation products with lower m / z with CFE from B. subtilis after 96 h. Less toxic AFB1 derivative detected after incubation with CFE from B. subtilis.