Chemical study, antioxidant activity, and genotoxicity and cytotoxicity evaluation of Ruellia angustiflora. Issue 23 (2nd December 2021)

• Record Type: Journal Article
• Authors: Pires, Fernanda B.; Dolwitsch, Carolina B.; Ugalde, Gustavo A.; Menezes, Bryan B.; Fontana, Marlos Eduardo Z.; Rieffeld, Roberta C.; Sagrillo, Michele R.; Essi, Liliana; Mazutti, Marcio Antonio; da Rosa, Marcelo B.; Pizzutti, Ionara Regina
• Is Part Of: Natural product research. Volume 35:Issue 23(2021)
• Page Start: 5317
• Page End: 5322
• Publication Date: 2021-12-02
• Subjects: Chemical characterization -- ultrasound-assisted extraction -- supercritical fluid extraction -- antioxidant activity -- cyto-genotoxicity -- Ruellia angustiflora; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2020.1753732
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Ruellia angustiflora (Acanthaceae) is known as flower-of-fire, and its leaves are traditionally employed to promote wound healing. This study was aimed at extracting and characterizing the chemical constituents of the extracts of R. angustiflora obtained by ultrasound-assisted and supercritical fluid extractions, and subsequently investigate their antioxidant potential and cyto-genotoxicity. The extract obtained by ultrasound (UAE-EtOH) was characterized by ultraperformance liquid chromatography-mass spectrometer (UPLC-MS), and the extract obtained via supercritical fluid (SFE-CO2 ) by gas chromatograph-mass spectrometer (GC-MS). The antioxidant potential was verified by the antiradical activity against the 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), and the cyto-genotoxicity evaluation to test cell viability and DNA damage was performed in peripheral blood mononuclear cells (PBMC) cultures. The SFE-CO2 extract presented some fatty acids, triterpenes, tetraterpenes, tocopherols and phytosterols. The UAE-EtOH extract contained phenolic acids and flavonoids, and showed the highest antioxidant capacity. Neither extract was genotoxic or cytotoxic at the tested concentrations. Graphical Abstract: