An IncP-2 plasmid sublineage associated with dissemination of blaIMP-45 among carbapenem-resistant Pseudomonas aeruginosa. Issue 1 (1st January 2021)

• Record Type: Journal Article
• Authors: Zhang, Xuefei; Wang, Leilei; Li, Dan; Li, Pei; Yuan, Lili; Yang, Fan; Guo, Qinglan; Wang, Minggui
• Is Part Of: Emerging microbes & infections. Volume 10:Issue 1(2021)
• Page Start: 442
• Page End: 449
• Publication Date: 2021-01-01
• Subjects: Carbapenem-resistant Pseudomonas aeruginosa -- outbreak -- blaIMP-45 -- IncP-2 plasmid -- sublineage -- dissemination; Medical microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.9041
• DOI: 10.1080/22221751.2021.1894903
• Languages: English
• ISSNs: 2222-1751

Abstract

ABSTRACT: IMP-45, a variant of IMP-9, is one of the dominant metallo-β-lactamases (MBLs) in clinical carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates in China. The aim of this study was to investigate the distribution and mechanism of dissemination of bla IMP-45 . MBL genes were detected by PCR in 173 non-duplicate CRPA isolates collected from Hospital HS in Shanghai and 605 P. aeruginosa isolates from a multicenter surveillance of bla IMP-45 in China. In total, 17 IMP-45-producers (14 from Hospital HS and 3 from other hospitals) were identified. Molecular typing identified an outbreak of 11 IMP-45-producing ST508 CRPA in the ICU of Hospital HS. Conjugation assays and whole genome sequencing were conducted among IMP-45-producers. Genomic comparison revealed that 16 bla IMP-45 -carrying plasmids (9 from this study and 7 from GenBank) shared a similar backbone with IncP-2 bla IMP-9 -carrying plasmid pOZ176 but lacked repA - oriV - parAB region. repA2 gene was presented in pOZ176, bla IMP-45 -carrying plasmids (17 from this study and 7 from GenBank) and 15 megaplasmids from GenBank. Phylogenetic analysis of repA2 showed that most bla IMP-45 -carrying plasmids were clustered into a sublineage separate from the one containing pOZ176. This IncP-2 plasmid sublineage contributed to the dissemination of bla IMP-45 among genetically diverse P. aeruginosa and recruited multiple resistance genes during its evolution.