Magnetically active pNIPAM nanosystems as temperature-sensitive biocompatible structures for controlled drug delivery. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Garcia-Pinel, Beatriz; Ortega-Rodríguez, Alicia; Porras-Alcalá, Cristina; Cabeza, Laura; Contreras-Cáceres, Rafael; Ortiz, Raul; Díaz, Amelia; Moscoso, Ana; Sarabia, Francisco; Prados, José; López-Romero, Juan M.; Melguizo, Consolación
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 48:Issue 1(2020)
• Page Start: 1022
• Page End: 1035
• Publication Date: 2020-01-01
• Subjects: pNIPAM nanosystems -- magnetic nanoparticles -- 5-fluorouracil -- oxaliplatin -- colon cancer -- external magnetic field; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.1080/21691401.2020.1773488
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract: Here, temperature-sensitive hybrid poly(N-isopropylacrylamide) (pNIPAM) nanosystems with magnetic response are synthesised and investigated for controlled release of 5-fluorouracil (5FU) and oxaliplatin (OXA). Initially, magnetic nanoparticles (@Fe3 O4 ) are synthesised by co-precipitation approach and functionalised with acrylic acid (AA), 3-butenoic acid (3BA) or allylamine (AL) as comonomers. The thermo-responsive polymer is grown by free radical polymerisation using N-isopropylacrylamide (NIPAM) as monomer, N, N'-methylenbisacrylamide (BIS) as cross-linker, and 2, 2'-azobis(2-methylpropionamidene) (V50) as initiator. We evaluate particle morphology by transmission electron microscopy (TEM) and particle size and surface charge by dynamic light scattering (DLS) and Z-potential (ZP) measurements. These magnetically active pNIPAM@ nanoformulations are loaded with 5-fluorouracil (5FU) and oxaliplatin (OXA) to determine loading efficiency, drug content and release as well as the cytotoxicity against T-84 colon cancer cells. Our results show high biocompatibility of pNIPAM nanoformulations using human blood cells and cultured cells. Interestingly, the pNIPAM@Fe3 O4 -3BA + 5FU nanoformulation significantly reduces the growth of T-84 cells (57% relative inhibition of proliferation). Indeed, pNIPAM-co-AL@Fe3 O4 -AA nanosystems produce a slight migration of HCT15 cells in suspension in the presence of an external magnetic field. Therefore, the obtained hybrid nanoparticles can be applied as a promising biocompatible nanoplatform for the delivery of 5FU and OXA in the improvement of colon cancer treatments. Graphical Abstract: UF0001