DNA Isolation from Mammalian Samples. Issue 1 (1st April 2013)

• Record Type: Journal Article
• Authors: Liu, Ximeng; Harada, Shuko
• Editors: Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin
• Is Part Of: Current protocols in molecular biology. Volume 102:Issue 1(2013)
• Page Start: 2.14.1
• Page End: 2.14.13
• Publication Date: 2013-04-01
• Subjects: DNA isolation -- silica spin column -- FFPE (formalin‐fixed paraffin‐embedded) tissue -- automated extraction; Molecular biology -- Technique -- Periodicals; Molecular biology -- Laboratory manuals; Molecular Biology -- methods; Biologie moléculaire -- Technique; Biologie moléculaire -- Manuels de laboratoire; Molecular biology; Molecular biology -- Technique; Laboratory Manual; Electronic reference sources; Laboratory manuals; 572.8028
• DOI: 10.1002/0471142727.mb0214s102
• Languages: English
• ISSNs: 1934-3639

Abstract

Abstract: DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica‐based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin‐fixed, paraffin‐embedded (FFPE) tissue samples. Curr. Protoc. Mol. Biol . 102:2.14.1–2.14.13. © 2013 by John Wiley & Sons, Inc.