Basic Image Analysis and Manipulation in ImageJ. Issue 1 (1st April 2013)

• Record Type: Journal Article
• Authors: Hartig, Sean M.
• Editors: Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin
• Is Part Of: Current protocols in molecular biology. Volume 102:Issue 1(2013)
• Page Start: 14.15.1
• Page End: 14.15.12
• Publication Date: 2013-04-01
• Subjects: ImageJ -- microscopy -- image analysis -- object identification -- spectral unmixing; Molecular biology -- Technique -- Periodicals; Molecular biology -- Laboratory manuals; Molecular Biology -- methods; Biologie moléculaire -- Technique; Biologie moléculaire -- Manuels de laboratoire; Molecular biology; Molecular biology -- Technique; Laboratory Manual; Electronic reference sources; Laboratory manuals; 572.8028
• DOI: 10.1002/0471142727.mb1415s102
• Languages: English
• ISSNs: 1934-3639

Abstract

Abstract: Image analysis methods have been developed to provide quantitative assessment of microscopy data. In this unit, basic aspects of image analysis are outlined, including software installation, data import, image processing functions, and analytical tools that can be used to extract information from microscopy data using ImageJ. Step‐by‐step protocols for analyzing objects in a fluorescence image and extracting information from two‐color tissue images collected by bright‐field microscopy are included. Curr. Protoc. Mol. Biol . 102:14.15.1–14.15.12. © 2013 by John Wiley & Sons, Inc.