Utilization of xylan-type polysaccharides in co-culture fermentations of Bifidobacterium and Bacteroides species. (15th May 2020)

• Record Type: Journal Article
• Authors: Zeybek, Nuket; Rastall, Robert A.; Buyukkileci, Ali Oguz
• Is Part Of: Carbohydrate polymers. Volume 236(2020)
• Publication Date: 2020-05-15
• Subjects: SCFA short-chain fatty acids -- XOS xylooligosaccharides -- AX arabinoxylan -- AXOS arabinoxylooligosaccharides; Dietary polysaccharides -- Xylan -- Prebiotics -- Bifidobacteria -- Bacteroides -- Cross-feeding; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116076
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Xylan is a widely available carbohydrate with prebiotic potential. Among five Bifidobacterium species, none could grow on xylan in pure cultures. Only B. animalis subsp. lactis utilized XOS in pure cultures. B. animalis subsp. lactis could grow on xylan in a co-culture with Bacteroides species. Results indicate a cross-feeding between Bacteroides and Bifidobacterium . Abstract: Although most members of the genus Bifidobacterium are unable to utilize xylan as a carbon source, the growth of these species can be induced by this polysaccharide in the gut environment. This indicates a requirement for an association between Bifidobacterium species and some other members of gut microbiota. In this study, the role of cross-feeding between Bifidobacterium and Bacteroides species in the bifidogenic effect of xylan was investigated using in-vitro pure and co-culture fermentations. The pure culture studies showed that among the Bifidobacterium species tested, only Bifidobacterium animalis subsp. lactis was able to utilize xylooligosaccharides. The co-culture of this strain with Bacteroides species enabled it to grow in the presence of xylan. These results suggest that the ability of Bacteroides species to hydrolyze xylan could allow the proliferation of specific Bifidobacterium species in the gut through substrate cross-feeding.