Chemical composition, cytotoxicity and molecular profiling of Cordia africana Lam. on human breast cancer cell line. Issue 21 (2nd November 2021)

• Record Type: Journal Article
• Authors: Ashmawy, Abeer M.; Ayoub, Iriny M.; Eldahshan, Omayma A.
• Is Part Of: Natural product research. Volume 35:Issue 21(2021)
• Page Start: 4133
• Page End: 4138
• Publication Date: 2021-11-02
• Subjects: Cordia africana -- cytotoxicity -- MCF-7 -- BcL-2 -- Ki-67 -- caspase-8 -- apoptosis; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2020.1736064
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Metabolic profiling of the hexane extract and essential oil of Cordia africana Lam., an economically important agroforestry species, was performed using GC-FID and GC-MS. Cytotoxicity was assessed in MCF-7 cancer cells using sulphorhodamine-B assay. Caspase-8, BcL-2 and Ki-67 levels were evaluated using enzyme-linked immunosorbent assay. Forty-one compounds were identified in the hexane extract, meanwhile, eighty compounds were annotated in the essential oil. n-Decane (9.52%), β-sitosterol (8.13%), undecane (6.32%) and palmitaldehyde (5.50 %) were the major compounds identified in the hexane extract, whereas, β-caryophyllene (32.0%), germacrene D (10.12%) and δ-cadinene (7.20%) predominated in the essential oil. The half maximal inhibitory concentrations (IC50 ) of the extract and oil were 4.55 µg/mL and 12.90 µg/mL, respectively. Caspase-8 activity increased significantly in hexane extract treated cells. Furthermore, BcL-2 and Ki-67 protein expression levels were significantly decreased. Cordia africana Lam exhibit potent cytotoxicity against breast cancer cell line, most likely through apoptosis regulation. Abstract :