Deformability Calculation for Estimation of the Relative Stability of Chemically Modified RNA Duplexes. Issue 1 (2nd March 2017)

• Record Type: Journal Article
• Authors: Masaki, Yoshiaki; Sekine, Mitsuo; Seio, Kohji
• Editors: Egli, Martin; Herdewijn, Piet; Matusda, Akira; Sanghvi, Yogesh S.
• Is Part Of: Current protocols in nucleic acid chemistry. Volume 68:Issue 1(2017)
• Page Start: 7.27.1
• Page End: 7.27.10
• Publication Date: 2017-03-02
• Subjects: deformability -- nucleic acid -- duplex thermal stability -- chemical modification -- RNA; Nucleic acids -- Laboratory manuals; Nucleic Acids -- chemical synthesis; Nucleic acids; Laboratory Manuals; Electronic books; Laboratory manuals; 572.8028
• DOI: 10.1002/cpnc.25
• Languages: English
• ISSNs: 1934-9270

Abstract

Abstract: Chemical modification of RNA duplexes alters their stability. We have attempted to develop a computational approach to estimate the thermal stability of chemically modified duplexes. These studies revealed that the deformability of chemically modified RNA duplexes, calculated from molecular dynamics simulations, could be used as a good indicator for estimating the effect of chemical modification on duplex thermal stability. This unit describes how deformability calculation can be applied to estimate the relative stability of chemically modified RNA duplexes. © 2017 by John Wiley & Sons, Inc.