Compartmentalized Self‐Tagging for In Vitro‐Directed Evolution of XNA Polymerases. Issue 1 (24th June 2014)

• Record Type: Journal Article
• Authors: Pinheiro, Vitor B.; Arangundy‐Franklin, Sebastian; Holliger, Philipp
• Editors: Egli, Martin; Herdewijn, Piet; Matusda, Akira; Sanghvi, Yogesh S.
• Is Part Of: Current protocols in nucleic acid chemistry. Volume 57:Issue 1(2014)
• Page Start: 9.9.1
• Page End: 9.9.18
• Publication Date: 2014-06-24
• Subjects: DNA polymerases -- in vitro‐directed evolution -- synthetic nucleic acids -- XNA -- compartmentalized self‐tagging -- CST; Nucleic acids -- Laboratory manuals; Nucleic Acids -- chemical synthesis; Nucleic acids; Laboratory Manuals; Electronic books; Laboratory manuals; 572.8028
• DOI: 10.1002/0471142700.nc0909s57
• Languages: English
• ISSNs: 1934-9270

Abstract

Abstract: Template‐dependent synthesis of xenobiotic nucleic acids (XNAs) is an essential step for the development of functional XNA molecules, as it enables Darwinian evolution to be carried out with novel genetic polymers. The extraordinary substrate specificity of natural DNA polymerases greatly restricts the spectrum of XNAs available, thus making it necessary to identify DNA polymerase variants capable of incorporating a wider range of substrates. This unit summarizes compartmentalized self‐tagging (CST), a directed evolution strategy developed for the selection of DNA polymerase variants capable of XNA synthesis. Curr. Protoc. Nucleic Acid Chem . 57:9.9.1‐9.9.18. © 2014 by John Wiley & Sons, Inc.