Circular RNA 0102049 suppresses the progression of osteosarcoma through modulating miR-520g-3p/PLK2 axis. Issue 1 (1st January 2021)

• Record Type: Journal Article
• Authors: Zhang, Xianliao; Hu, Zhengbo; Li, Wenhu; Liu, Zhongxun; Li, Jie; Wang, Zhaozhen; Martin, Vidmi Taolam; Yan, Bing; Yu, Bo
• Is Part Of: Bioengineered. Volume 12:Issue 1(2021)
• Page Start: 2022
• Page End: 2032
• Publication Date: 2021-01-01
• Subjects: Osteosarcoma -- circ_0102049 -- miR-520g-3p -- PLK2; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2021.1923259
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: Circular RNAs (circRNAs) are a type of non-coding RNAs generated from back splicing to enhance or inhibit the progression of multiple human cancers including osteosarcoma (OS). Although circ_0102049 has been found to be highly expressed in OS cell lines, the role and specific mechanism of circ_0102049 in OS remains unclear. Here, we found that silence of circ_0102049 could significantly exacerbate the tumorigenesis of OS in vivo through sponging microRNA-520g-3p. Polo-like kinase 2 (PLK2) was predicted to be a target of miR-520g-3p, and luciferase reporter assay revealed that overexpression of miR-520g-3p dramatically suppressed the expression of PLK2, whereas miR-520g-3p inhibitor promoted the PLK2 expression. Moreover, the silence of circ_0102049 could markedly promote the proliferation, invasion, migration and cell-cycle promotion while inhibiting the apoptosis of OS cell line MG63 cells in vitro through regulating miR-520g-3p/PLK2 axis. Taken together, the present study indicated that circ_0102049 suppressed the progression of osteosarcoma via modulating miR-520g-3p/PLK2/TAp73 axis, providing a potential therapeutic target for OS. Graphical abstract: uf0001