How to Build a Time‐Gated Luminescence Microscope. Issue 1 (16th February 2018)

• Record Type: Journal Article
• Authors: Jin, Dayong; Lu, Yiqing; Leif, Robert C.; Yang, Sean; Rajendran, Megha; Miller, Lawrence W.
• Editors: Robinson, J. Paul; Darzynkiewicz, Zbigniew; Hoffman, Robert; Nolan, John P.; Rabinovitch, Peter S.; Watkins, Simon
• Is Part Of: Current protocols in cytometry. Volume 67:Issue 1(2014)
• Page Start: 2.22.1
• Page End: 2.22.36
• Publication Date: 2018-02-16
• Subjects: lanthanide -- time‐gated luminescence -- microscopy -- autofluorescence; Cytology -- Laboratory manuals; Flow cytometry -- Laboratory manuals; Cell separation -- Laboratory manuals; Molecular biology -- Laboratory manuals; Flow Cytometry -- methods; Image Cytometry -- methods; Cell Separation -- methods; Cytological Techniques; Molecular Biology -- methods; Cell separation; Cytology; Flow cytometry; Molecular biology; Laboratory Manuals; Laboratory manuals; 571.6
• DOI: 10.1002/0471142956.cy0222s67
• Languages: English
• ISSNs: 1934-9297

Abstract

Abstract: The sensitivity of filter‐based fluorescence microscopy techniques is limited by autofluorescence background. Time‐gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time‐gated luminescence microscopes. Three detailed, step‐by‐step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time‐gated luminescence. Curr. Protoc. Cytom . 67:2.22.1‐2.22.36. © 2014 by John Wiley & Sons, Inc.