Quantification of Th1 and Th17 Cells with Intracellular Staining Following PMA/Ionomycin Stimulation. Issue 1 (5th January 2015)

• Record Type: Journal Article
• Authors: Karlsson, Fridrik; Hassan‐Zahraee, Mina
• Editors: Robinson, J. Paul; Darzynkiewicz, Zbigniew; Hoffman, Robert; Nolan, John P.; Rabinovitch, Peter S.; Watkins, Simon
• Is Part Of: Current protocols in cytometry. Volume 71:Issue 1(2015)
• Page Start: 6.35.1
• Page End: 6.35.7
• Publication Date: 2015-01-05
• Subjects: Th1 cells -- Th17 cells -- intracellular staining -- flow cytometry -- adaptive immunity; Cytology -- Laboratory manuals; Flow cytometry -- Laboratory manuals; Cell separation -- Laboratory manuals; Molecular biology -- Laboratory manuals; Flow Cytometry -- methods; Image Cytometry -- methods; Cell Separation -- methods; Cytological Techniques; Molecular Biology -- methods; Cell separation; Cytology; Flow cytometry; Molecular biology; Laboratory Manuals; Laboratory manuals; 571.6
• DOI: 10.1002/0471142956.cy0635s71
• Languages: English
• ISSNs: 1934-9297

Abstract

Abstract: Cytokine‐producing cells are at the center of the adaptive immune responses, and quantifying these cells is an important aspect to build understanding of the immune response. In particular, Th1 and Th17 cells have been implicated in the pathogenesis of such diseases as inflammatory bowel disease, rheumatoid arthritis, and multiple sclerosis. Quantification of Th1 and Th17 cells can provide important information in research of these diseases and other Th1‐ and Th17‐mediated immune disorders. In vitro stimulation of cells followed by surface and intracellular staining, presented here, has the advantage of detecting the cytokines directly instead of relying exclusively on surrogate surface markers which, although showing enrichment for the effector T cells, are not specific markers for the cytokine‐producing cells. © 2015 by John Wiley & Sons, Inc.