Instability of the Pseudoautosomal Boundary in House Mice. Issue 2 (26th April 2019)

• Record Type: Journal Article
• Authors: Morgan, Andrew P; Bell, Timothy A; Crowley, James J; Pardo-Manuel de Villena, Fernando
• Is Part Of: Genetics. Volume 212:Issue 2(2019)
• Page Start: 469
• Page End: 487
• Publication Date: 2019-04-26
• Subjects: meiosis -- recombination -- pseudoautosomal region -- sex chromosome evolution; Genetics -- Periodicals; 576.5
• DOI: 10.1534/genetics.119.302232
• Languages: English
• ISSNs: 0016-6731

Abstract

Abstract: Faithful segregation of mammalian X and Y chromosomes in male meiosis depends on pairing and recombination in a short interval of residual sequence homology known as the pseudoautosomal region (PAR)... Faithful segregation of homologous chromosomes at meiosis requires pairing and recombination. In taxa with dimorphic sex chromosomes, pairing between them in the heterogametic sex is limited to a narrow interval of residual sequence homology known as the pseudoautosomal region (PAR). Failure to form the obligate crossover in the PAR is associated with male infertility in house mice ( Mus musculus ) and humans. Yet despite this apparent functional constraint, the boundary and organization of the PAR is highly variable in mammals, and even between subspecies of mice. Here, we estimate the genetic map in a previously documented expansion of the PAR in the M. musculus castaneus subspecies and show that the local recombination rate is 100-fold higher than the autosomal background. We identify an independent shift in the PAR boundary in the M. musculus musculus subspecies and show that it involves a complex rearrangement, but still recombines in heterozygous males. Finally, we demonstrate pervasive copy-number variation at the PAR boundary in wild populations of M. m. domesticus, M. m. musculus, and M. m. castaneus . Our results suggest that the intensity of recombination activity in the PAR, coupled with relatively weak constraints on its sequence, permit the generation and maintenance of unusual levels of polymorphism in the population of unknown functional significance.