Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities. (15th May 2020)

• Record Type: Journal Article
• Authors: Hadidi, Milad; Pouramin, Shiva; Adinepour, Fateme; Haghani, Shaghayegh; Jafari, Seid Mahdi
• Is Part Of: Carbohydrate polymers. Volume 236(2020)
• Publication Date: 2020-05-15
• Subjects: Clove essential oil -- Nanoencapsulation -- Nanoparticles -- Chitosan -- Antibacterial activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116075
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Clove essential oil (CEO) was encapsulated into chitosan nanoparticles. FTIR, X-ray diffraction and DSC confirmed the successful loading of CEO. Sizes of CEO-loaded nanoparticles ranged from 223 to 444 nm. Encapsulation improved the antioxidant and antibacterial activity of CEO. Abstract: One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8–73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223−444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80–4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.