In-vitro antitumor activity of compounds from Glycyrrhiza glabra against C6 glioma cancer cells: identification of natural lead for further evaluation. Issue 23 (2nd December 2021)

• Record Type: Journal Article
• Authors: Goel, Bharat; Sharma, Anuradha; Tripathi, Nancy; Bhardwaj, Nivedita; Sahu, Bharat; Kaur, Gurcharan; Singh, Bikarma; Jain, Shreyans K.
• Is Part Of: Natural product research. Volume 35:Issue 23(2021)
• Page Start: 5489
• Page End: 5492
• Publication Date: 2021-12-02
• Subjects: Glycyrrhiza glabra -- C6 glioma cells -- cytotoxicity -- bioassay guided isolation -- glycyrrhetic acid; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2020.1786830
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: A HP20 resin-based unique method was adopted to get an active fraction of the hydroalcoholic extract of G. glabra roots. The fraction showed potent cytotoxicity against cancer cell line and was further subjected to detailed phytochemical investigation to obtain ten biomarkers. The isolated compounds were also tested for the cytotoxicity against the C6 glioma cell line in vitro using MTT assay. Among the isolated compounds, glycyrrhetic acid (1 ), glabrol (6 ), and glabridin (9 ) exhibited significant cytotoxicity. The compounds showed a dose-dependent decrease in cell viability. The active compounds were subjected to molecular docking study against topoisomerase I and topoisomerase II to support the mechanism of antitumor activity. Graphical Abstract: UF0001