Research methodology for in vivo measurements of resting energy expenditure, daily body temperature, metabolic heat and non-viral tissue-specific gene therapy in baboons. (December 2020)

• Record Type: Journal Article
• Authors: Frost, Patrice A.; Chen, Shuyuan; Rodriguez-Ayala, Ernesto; Laviada-Molina, Hugo A.; Vaquera, Zoila; Gaytan-Saucedo, Janeth F.; Li, Wen-Hong; Haack, Karin; Grayburn, Paul A.; Sayers, Ken; Cole, Shelley A.; Bastarrachea, Raul A.
• Is Part Of: Research in veterinary science. Volume 133(2020)
• Page Start: 136
• Page End: 145
• Publication Date: 2020-12
• Subjects: Baboons -- Data loggers -- Indirect calorimetry -- Metabolic heat -- Veterinary infrared thermography -- UTMD -- Gene therapy; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2020.09.020
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: A large number of studies have shown that the baboon is one of the most commonly used non-human primate (NHP) research model for the study of immunometabolic complex traits such as type 2 diabetes (T2D), insulin resistance (IR), adipose tissue dysfunction (ATD), dyslipidemia, obesity (OB) and cardiovascular disease (CVD). This paper reports on innovative technologies and advanced research strategies for energetics and translational medicine with this NHP model. This includes the following: measuring resting energy expenditure (REE) with the mobile indirect calorimeter Breezing®; monitoring daily body temperature using subcutaneously implanted data loggers; quantifying metabolic heat with veterinary infrared thermography (IRT) imaging, and non-viral non-invasive, tissue-specific ultrasound-targeted microbubble destruction (UTMD) gene-based therapy. These methods are of broad utility; for example, they may facilitate the engineering of ectopic overexpression of brown adipose tissue (BAT) mUCP-1 via UTMD-gene therapy into baboon SKM to achieve weight loss, hypophagia and immunometabolic improvement. These methods will be valuable to basic and translational research, and human clinical trials, in the areas of metabolism, cardiovascular health, and immunometabolic and infectious diseases. Graphical abstract: Unlabelled Image Highlights: Baboons are an outstanding NHP model for immunometabolic research. Innovative technologies for energetics and translational medicine with this NHP model are reported. Methods developed to measure REE (indirect calorimeter) and temperature (thermologgers and infrared imaging). We engineered SKM cells into a BAT phenotype through UTMD technology in baboons.