Janus electro-microenvironment membrane with surface-selective osteogenesis/gingival healing ability for guided bone regeneration. (15th December 2022)

• Record Type: Journal Article
• Authors: Lai, Chunhua; Cheng, Mingwei; Ning, Chengyun; He, Yiheng; Zhou, Zhengnan; Yin, Zhaoyi; Zhu, Peijun; Xu, Yan; Yu, Peng; Xu, Shulan
• Is Part Of: Materials today bio. Volume 17(2023)
• Publication Date: 2022-12-15
• Subjects: Electric microenvironment -- Piezoelectric -- Guided bone regeneration -- Osteogenic -- Janus membrane; Materials science -- Periodicals; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 620.1
• DOI: 10.1016/j.mtbio.2022.100491
• Languages: English
• ISSNs: 2590-0064

Abstract

Abstract: Guided bone regeneration is widely applied in clinical practice to treat alveolar bone defects. However, the rate of healing of severe alveolar bone defects is slow, and there is a high incidence of soft tissue wound dehiscence. In this study, we propose a barrier membrane with a Janus electro-microenvironment (JEM) to achieve side-selective bone regeneration and soft tissue healing. The JEM membrane was constructed using a polarized polyvinylidene fluoride ferroelectric membrane with different surface potentials on either side. It promoted osteogenic differentiation and bone regeneration on the negatively polarized side (JEM-) and soft tissue regeneration on the positively polarized side (JEM+). Further investigation revealed that the JEM-mediated promotion of bone formation was related to mitochondrial autophagy, as indicated by depolarization of the mitochondrial membrane potential and the expression of LC3, Pink I, and Parkin. Moreover, the gingival healing promoted by JEM+ was related to oxidative phosphorylation in mitochondria, as indicated by the upregulation of mitochondrial complexes I–V and an increase in ATP generation. The design concept of the JEM provides a new avenue for regulating tissue regeneration between different tissue interfaces. Graphical abstract: Schematic 1. Schematic illustration of the Janus electro-microenvironment (JEM) membrane . JEM based on a polarized poly(vinylidene fluoride-co-trifluoroethylene) (P(VDF-TrFE)) membrane to promote surface-selective osteogenesis/gingival healing. The direction of electrical dipoles of the P(VDF-TrFE) membrane is reoriented after external electric field polarization, resulting in the formation of a JEM membrane due to surface potential transition on the different surfaces of the ferroelectric P(VDF-TrFE) membrane. The electro-microenvironment of the positively polarized side of the P(VDF-TrFE) membrane is denoted as JEM(+) and the electro-microenvironment of the negatively polarized side is denoted as JEM(−). When the JEM membrane is placed between the bone defect and gingival tissue defect, JEM(+) promotes soft tissue regeneration by stimulating mitochondrial oxidative phosphorylation, which could produce ATP and provide energy for cellular metabolism and biosynthesis, while JEM(−) promotes bone tissue regeneration by stimulating mitophagy, which could speed up mineral transportation and deposition. The short red arrows denote the direction of electrical dipole in the JEM membrane. Dotted lines denote the boundaries of the new soft tissue formed. Image 1