Schinus terebinthifolius fruits intake ameliorates metabolic disorders, inflammation, oxidative stress, and related vascular dysfunction, in atherogenic diet-induced obese rats. Insight of their chemical characterization using HPLC-ESI-QTOF-MS/MS. (6th April 2021)

• Record Type: Journal Article
• Authors: Feriani, Anouar; Tir, Meriam; Arafah, Maria; Gómez-Caravaca, Ana María; Contreras, María del Mar; Nahdi, Saber; Taamalli, Amani; Allagui, Mohamed Salah; Alwasel, Saleh; Segura-Carretero, Antonio; Harrath, Abdel Halim; Tlili, Nizar
• Is Part Of: Journal of ethnopharmacology. Volume 269(2021)
• Publication Date: 2021-04-06
• Subjects: S. terebinthifolius -- HPLC-ESI-QTOF-MS/MS -- Inflammatory -- Oxidative stress -- Glucid and lipid profile -- Atherogenic; Alanine aminotransferase (ALT) -- Aspartate amino-transferase (AST) -- Atherogenic Diet (AD) -- Catalase (CAT) -- C-reactive protein (CRP) -- HDL-cholesterol (HDL-C) -- Glutathione peroxidase (GPx) -- High-performance liquid chromatography with diode array detector coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC-DAD-ESI-QTOF-MS) -- Interleukin-6 (IL-6) -- Lactate dehydrogenase (LDH) -- LDL-cholesterol (LDL-C) -- Malondialdehyde (MDA) -- Protein carbonyls (PC) -- Superoxide dismutase (SOD) -- S. terebinthifolius fruit extract (STFE) -- Total cholesterol (TC) -- Triglycerides (TG) -- Tumor necrosis factor (TNF-α); Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2020.113701
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacology relevance: Schinus terebinthifolius is traditionally used for its anti inflammatory capacity, and indicated as a cardioprotective agent, whereas, its preventive effect against atherogenic diet fed (AD) induced metabolic disorders and the underlying mechanisms has not yet been explored. Aim of the study: This study was undertaken to investigate the ameliorative role of Schinus terebinthifolius fruits extract (STFE) against cardiovascular problem, oxidative and inflammatory status related to obesity in rats fed an atherogenic diet. Materials and methods: The metabolites profile in STFE was evaluated using HPLC-DAD-ESI-QTOF-MS/MS analysis. In Wistar rats, atherogenic diet was added for 9 weeks to induce lipid accumulation simultaneously with STFE (50 mg/kg b. w) or saline treatment. Biochemical, oxidant, and inflammatory criteria together with hepatic and arterial integrity examination were assessed. Results: A total of thirty three metabolites were identified using HPLC-DAD-ESI-QTOF-MS, among them masazino-flavanone was the major compound (2645.50 μg/g DW). The results indicated that STFE supplementation during 9 weeks (50 mg/kg b. w.) significantly attenuated the altered lipid profile by decreasing the levels of TC, TG, LDL-C and increasing the HDL-C content both in plasma and liver, when compared with the AD-group. The histological analysis using ORO staining revealed a decrease in the lipid droplet deposit in the cytoplasm of hepatocytes of STFE + AD group. The addition of STFE could improve the glycemic status of AD-treated rats by decreasing the glucose and insulin secretion, and ameliorating the hepatic glycogen synthesis. The harmful effects of atherogenic diet on hepatic oxidative stress indicators (MDA, PC, GSH, SOD, CAT, and GPx), biochemical markers (AST, ALT, LDH and ALP), and liver function, were found to be decreased by the addition of STFE. Moreover, the reduction of inflammatory markers (CRP, IL-6 and TNF-α), associated to alleviating of aortic oxidative stress and integrity, highlighted the positive anti-atherogenic effect of STFE. Conclusion: Overall, the pleiotropic protective effect observed with S. terebinthifolius fruits might be related to the presence of various bioactive compounds. Graphical abstract: Image 1 Highlights: RP-HPLC-DAD-ESI-QTOF-MS/MS analysis of Schinus terentibofolis extract was performed. Thirty three phenolics molecules were identified and quantified. The preventive capacity of Shinus terentibofolis against atherogenic diet-induced metabolic disorders was explored. S. terentibofolis has antihyperlipidemic, anti-diabetic, antioxidative, anti-inflammatory, anti-atherogenic properties.