Increasing the antinociceptive effect of ingested glycinamide in female rats by increasing its palatability. (15th October 2020)

• Record Type: Journal Article
• Authors: Gómora-Arrati, Porfirio; González-Flores, Oscar; Morales-Medina, Julio César; Komisaruk, Barry
• Is Part Of: Neuroscience letters. Volume 737(2020)
• Publication Date: 2020-10-15
• Subjects: antinociception -- glycinamide ingestion -- chocolate -- glycine; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2020.135314
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Glycinamide-in-chocolate suspension induced antinociception in two nociceptive tests. Glycinamide induced antinociception most likely by increasing glycine levels. Abstract: Background: These studies were undertaken to investigate whether the ingestion of glycinamide, a precursor of glycine, made more palatable by mixing with a chocolate suspension, improves antinociception in rats. Methods: Two nociception threshold models were employed: the tail-flick latency and vocalization to tail shock, in restricted and freely-moving rats. Glycinamide in a highly palatable commercial chocolate aqueous suspension was provided for ad-lib ingestion after 24 hours of water deprivation. Antinociception threshold testing was performed before and 10, 30, 60, 90, 120, 180, and 240 minutes after the ingestion of the chocolate-glycinamide mixture. Results: Ingestion of the glycinamide-in-chocolate suspension induced antinociception based on the tail shock vocalization and tail-flick latency tests. Ingestion of the glycinamide-in-chocolate suspension induced an 80% elevation in the antinociceptive threshold that persisted for 4 hours. Conclusions: Rats readily ingest the glycine precursor, glycinamide, in an aqueous chocolate mixture, which induces potent and prolonged antinociception.