Application of a polyelectrolyte complex based on biocompatible polysaccharides for colorectal cancer inhibition. (January 2021)

• Record Type: Journal Article
• Authors: Souza, Paulo R.; Vilsinski, Bruno H.; Nunes, Cátia S.; Bonkovoski, Letícia C.; Garcia, Francielle; Nakamura, Celso V.; Caetano, Wilker; Valente, Artur J.M.; Martins, Alessandro F.; Muniz, Edvani C.
• Is Part Of: Carbohydrate research. Volume 499(2021)
• Publication Date: 2021-01
• Subjects: Chloroaluminum phthalocyanine -- Ionic liquid -- Chondroitin sulphate -- Chitosan -- Photodynamic therapy; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2020.108194
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Strategies for incorporating water-insoluble photosensitisers (PS) in drug delivery systems have been extensively studied. In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene- block -poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc). The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The PS-b-PAA NPs on the PEC surface was confirmed by scanning electron microscopy (SEM). Additionally, optical images distinguished the PEC structures containing PS-b-PAA or PS-b-PAA/AlClPc from the unloaded PEC. Kinetic and equilibrium studies investigate the sorption capacity of the PEC/PS-b-PAA toward AlClPc. The encapsulation efficiency reached 95% at 190 μg mL −1 AlClPc after only 15 min. The Brunauer-Emmett-Teller (BET) isotherm and pseudo-second-order kinetic fitted well to the experimental data. The PS-b-PAA NPs on the PEC surfaces increase the AlClPc bioavailability and the PEC structure stabilizes the PS-b-PAA/AlClPc nanostructures. The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells). For the first time, we associate PS-b-PAA/AlClPc with a hydrophilic and cytocompatible polysaccharide matrix. We suggest the use of these materials in strategies to treat cancer by using photodynamic therapy. Graphical abstract: Image 1 Highlights: Polyelectrolyte complex (PECs) coacervates were created from polymer blends in an ionic liquid. Polystyrene- block -poly(acrylic acid) nanoparticles were adsorbed on the PEC surfaces. The polystyrene- block -poly(acrylic acid)/PEC pair was loaded with chloroaluminum phthalocyanine. We suggest the use of these materials to treat cancer using photodynamic therapy.