The Effect of Weight and Incubation Time in DNA Quality of Kepel (Stelechocarpus burahol) Leaves. Issue 1 (April 2021)

• Record Type: Journal Article
• Authors: Handayani, E; Nandariyah, ; Cahyani, V R; Parjanto,
• Is Part Of: IOP conference series. Volume 752:Issue 1(2021)
• Publication Date: 2021-04
• Subjects: DNA isolation -- kepel -- cetyl trimethyl ammonium bromide -- conservation; Earth sciences -- Periodicals; Environmental sciences -- Congresses; Environmental sciences -- Periodicals; 550.5
• DOI: 10.1088/1755-1315/752/1/012017
• Languages: English
• ISSNs: 1755-1307

Abstract

Abstract: Kepel ( Stelechocarpus burahol ) is a rare fruit plant which becomes the identity plant of the Yogyakarta Region. Kepel plants need to be conserved so they don't become extinct Germplasm of kepel must be collected to get the genetic diversity of Kepel . Research on the genetic diversity of Kepel plants has never been done, so it needs the right method to obtain good quality DNA for molecular studies for future research. This study aims to obtain the best sample weight and incubation time to produce high-quality DNA for future research. This study used samples from young green leaves of kepel . DNA was extracted and purified from the leaves using a modified CTAB method to obtain DNA of kepel with high-purity and high-concentration. The result of DNA isolation and purification was analyzed by spectrophotometer and agarose gel electrophoresis method. The yield of DNA isolation on young kepel leaves had higher quality and quantity than DNA isolation on mature kepel leaves The results demonstrated that DNA isolation from young green leaves of kepel had a purity level of 1.64 – 2.01. In addition, it also showed that DNA isolation from young green leaves in weight 0.2 g with 30 minutes incubation gave the highest DNA concentration (880 ng/µl).