An efficient heterologous Escherichia coli-based expression system for lectin production from Pleurocybella porrigens. Issue 3 (31st December 2020)

• Record Type: Journal Article
• Authors: Suzuki, Tomohiro; Nakamura, Luna; Inayoshi, Satomi; Tezuka, Yuki; Ono, Akiko; Choi, Jae-Hoon; Dohra, Hideo; Sasanami, Tomohiro; Hirai, Hirofumi; Kawagishi, Hirokazu
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 3(2021)
• Page Start: 630
• Page End: 633
• Publication Date: 2020-12-31
• Subjects: Heterologous expression -- lectin -- Pleurocybella porrigens -- Escherichia coli; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbaa058
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: In this study, we report a more efficient heterologous expression of lectin from Pleurocybella porrigens (PPL) using an Escherichia coli -based expression system. The yield (9.3 mg/L culture broth) of recombinant PPL (rPPL) using this expression system was increased approximately 9-fold compared to our previous study. The rPPL obtained in this study exhibited the same biochemical properties as the native PPL.