Genome of Superficieibacter maynardsmithii, a novel, antibiotic susceptible representative of Enterobacteriaceae. Issue 2 (13th February 2021)

• Record Type: Journal Article
• Authors: Batisti Biffignandi, Gherard; Gibbon, Marjorie J; Corbella, Marta; Thorpe, Harry A; Merla, Cristina; Castelli, Michele; Kallonen, Teemu; Pegrum, Katie; Brisse, Sylvain; Corander, Jukka; Marone, Piero; Feil, Edward J; Sassera, Davide
• Editors: Reyes, R
• Is Part Of: G3. Volume 11:Issue 2(2021)
• Publication Date: 2021-02-13
• Subjects: Superficieibacter -- Enterobacteriaceae -- ANI -- Hadamard; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1093/g3journal/jkab019
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: During a citywide microbiological screening project in Pavia (Italy) a bacterial strain isolated from the surface of an Automated Teller Machine was classified as a Klebsiella sp. by MALDI-TOF spectrometry, and shown to be susceptible to the most antimicrobial classes by phenotypic testing. After Illumina genome sequencing and subsequent assembly, a high-quality draft genome was obtained (size = 5, 051, 593 bp, N50 = 615, 571 bp, largest contig = 1, 328, 029 bp, N_contig = 17, GC content = 51.58%, coverage = 141.42), absence of antimicrobial resistance genes was confirmed, but the strain resulted to be highly divergent from all Klebsiella, and more related to other Enterobacteriaceae . The higher values of 16S rRNA identity were with members of the genera Citrobacter, Salmonella, and " Superficieibacter. " An ortholog-based phylogenomic analysis indicated a sister group relationship with " Superficieibacter electus, " in a distinct clade from other members of the Enterobacteriaceae family. In order to evaluate whether the novel genome represents a new species of " Superficiebacter, " average nucleotide identity (ANI) and Hadamard analysis were performed on a dataset of 78 Enterobacteriaceae . The novel genome showed an ANI of 87.51% with S. electus, which compared on identity values between other members of the family, clearly indicates that the genome represents a new species within the genus " Superficieibacter. " We propose for the new species the name " Superficieibacter maynardsmithii."