A Cre Transcription Fidelity Reporter Identifies GreA as a Major RNA Proofreading Factor in Escherichia coli. Issue 1 (1st May 2017)

• Record Type: Journal Article
• Authors: Bubunenko, Mikhail G; Court, Carolyn B; Rattray, Alison J; Gotte, Deanna R; Kireeva, Maria L; Irizarry-Caro, Jorge A; Li, Xintian; Jin, Ding J; Court, Donald L; Strathern, Jeffrey N; Kashlev, Mikhail
• Is Part Of: Genetics. Volume 206:Issue 1(2017)
• Page Start: 179
• Page End: 187
• Publication Date: 2017-05-01
• Subjects: RNA polymerase -- transcription errors -- Gre proteins -- Cre/lox fidelity reporter; Genetics -- Periodicals; 576.5
• DOI: 10.1534/genetics.116.198960
• Languages: English
• ISSNs: 0016-6731

Abstract

Abstract: We made a coupled genetic reporter that detects rare transcription misincorporation errors to measure RNA polymerase transcription fidelity in Escherichia coli . Using this reporter, we demonstrated in vivo that the transcript cleavage factor GreA, but not GreB, is essential for proofreading of a transcription error where a riboA has been misincorporated instead of a riboG. A greA mutant strain had more than a 100-fold increase in transcription errors relative to wild-type or a greB mutant. However, overexpression of GreB in Δ greA cells reduced the misincorporation errors to wild-type levels, demonstrating that GreB at high concentration could substitute for GreA in RNA proofreading activity in vivo .