Dual-acting antibacterial porous chitosan film embedded with a photosensitizer. Issue 1 (31st January 2020)

• Record Type: Journal Article
• Authors: Liu, Zi-Jun; Lin, Shu-Ching; Lee, Pei-Yuan; Lin, Ying-Ting; Lai, Zi-Lun; Chang, Cheng-Chung; Wang, Gou-Jen
• Is Part Of: Science and technology of advanced materials. Volume 21:Issue 1(2020)
• Page Start: 562
• Page End: 572
• Publication Date: 2020-01-31
• Subjects: Dual-acting antibacterial film -- porous chitosan/small molecular compound -- bactericidal and bacteriostatic effects; 102 Porous / Nanoporous / Nanostructured materials; 211 Scaffold / Tissue engineering/Drug delivery; Materials -- Technological innovations -- Periodicals; 620.112
• DOI: 10.1080/14686996.2020.1795431
• Languages: English
• ISSNs: 1468-6996

Abstract

ABSTRACT: This study proposes to develop a dual-acting antibacterial film of porous chitosan (Cs) embedded with small molecular compound, which possesses photosensitive characteristics with bactericidal efficacy, to promote the accelerated recovery of infectious wounds. The Cs/small molecular compound (Cs-cpd.2) dressing was prepared using the freeze-drying method. Characterization of the synthesized Cs-cpd.2 indicated that it has high porosity and moisture absorption effect, hence enhancing the absorption of wound exudate. Experimental results showed that Cs-cpd.2 dressing has good bactericidal and bacteriostatic effects on Staphylococcus aureus under visible-light irradiation and has antibacterial effect in the dark. It was also found that the small molecular compound does not have cytotoxicity at a dose of 0–5 μM. Furthermore, Cs-cpd.2 that contained small molecular compound with a concentration of 0.3–1 μM has positive effect on both the cell viability rate and cell proliferation rate of human fibroblast CG1639. Cs-cpd.2 can significantly promote cell proliferation when the small molecular compound and the basic fibroblast growth factor bFGF were added together. Therefore, the proposed Cs-cpd.2 dressing is feasible for photodynamic therapy (PDT) and clinical wound dressing applications. GRAPHICAL ABSTRACT: uf0001