Bionic design of cytochrome c oxidase-like single-atom nanozymes for oxygen reduction reaction in enzymatic biofuel cells. (May 2021)

• Record Type: Journal Article
• Authors: Zhang, He; Huang, Liang; Chen, Jinxing; Liu, Ling; Zhu, Xinyang; Wu, Weiwei; Dong, Shaojun
• Is Part Of: Nano energy. Volume 83(2021)
• Publication Date: 2021-05
• Subjects: Cytochrome c oxidase-like -- Single-atom nanozymes -- Oxygen reduction reaction -- Enzymatic biofuel cells; Nanoscience -- Periodicals; Nanotechnology -- Periodicals; Nanostructured materials -- Periodicals; Power resources -- Technological innovations -- Periodicals; Nanoscience; Nanostructured materials; Nanotechnology; Power resources -- Technological innovations; Periodicals; 621.042
• DOI: 10.1016/j.nanoen.2021.105798
• Languages: English
• ISSNs: 2211-2855

Abstract

Abstract: Designing an artificial enzyme, from the perspective of bionics, to mimic the catalytic activity of natural enzymes is highly desirable but remains challenges. In response to the simulation of biological structure, we developed cytochrome c oxidase (CcO)-like single-atom nanozymes with FeN5 active centers (FeN5 SAs) in this work. Similar to the spatial structure of heme a3 in natural CcO, the active center of FeN5 SAs is axial N-coordinated heme-like structure and can be served as oxygen-binding site to complete oxygen reduction reaction (ORR) in respiratory electron transport chain by catalyzing the oxidation of cytochrome c (Cyt c). Depending on this bionic structure, furthermore, FeN5 SAs exhibited competitive electrocatalytic performance towards ORR with a half-wave potential of 0.67 V (vs. RHE) at neutral condition. Coupling a glucose dehydrogenase (GDH) bioanode, the FeN5 SAs-based glucose/O2 enzymatic biofuel cell (EBFC) obtained a maximum power density of 149.2 ± 4.0 μW cm −2 with an open circuit potential of 0.40 ± 0.01 V. In this study, inspired by the native structure of enzymes, we develop CcO-like FeN5 SAs and expand its application in EBFCs, which may provide an rational research approach to advance the development of nanozymes. Graphical Abstract: Inspired by the native structure of enzymes, FeN5 SAs, with remarkably high cytochrome c oxidases-like activity (KM, 4.2 × 10 −5 M) and competitive electrocatalytic performance towards ORR ( E 1/2, 0.61 V, neutral condition), were developed in this work. Coupling a glucose dehydrogenase bioanode, the FeN5 SAs-based glucose/O2 EBFC obtained a maximum power density of 149.2 ± 4.0 μW cm −2 with an open circuit potential of 0.40 ± 0.01 V. ga1 Highlights: Inspired by the native structure of enzymes, CcO-like FeN5 single-atom nanozymes were developed in this work. FeN5 single-atom nanozymes display remarkably high CcO-like activity and competitive electrocatalytic performance towards ORR. The study of FeN5 single-atom nanozymes boosts the cross-merging between single-atom nanomaterials and artificial enzymes.