A novel enzyme synthesized by Acinetobacter sp. SM04 is responsible for zearalenone biodegradation. Issue 2 (1st December 2021)

• Record Type: Journal Article
• Authors: Tang, Yuqian; Liu, Chendi; Yang, Jiguo; Peng, Xian
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 86:Issue 2(2022)
• Page Start: 209
• Page End: 216
• Publication Date: 2021-12-01
• Subjects: Acinetobacter sp. SM04 -- biodegradation -- cloning -- enzyme -- zearalenone; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbab204
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Zearalenone (ZEA), a nonsteroidal estrogenic mycotoxin produced by multiple Fusarium species, contaminates cereals and threatens the health of both humans and animals by inducing hepatotoxicity, immunotoxicity, and genotoxicity. A new alkali tolerant enzyme named Ase, capable of degrading ZEA without H2 O2, was derived from Acinetobacter sp. SM04 in this study. The Ase gene shares 97% sequence identity with hypothetical proteins from Acinetobacter pittii strain WCHAP 100004 and YMC 2010/8/T346 and Acinetobacter calcoaceticus PHEA-2, respectively. Based on the Acinetobacter genus database, the gene encoding Ase was cloned and extracellularly expressed in Escherichia coli BL21. After degrading 88.4% of ZEA (20 µg/mL), it was confirmed through MCF-7 cell proliferation assays that Ase can transform ZEA into a nonestrogenic toxic metabolite. Recombinant Ase (molecular weight: 28 kDa), produced by E. coli BL21/pET32a(+)-His-Ase, was identified as an oxygen-utilizing and cytochrome-related enzyme with optimal activity at 60 °C and pH 9.0. Graphical Abstract: