Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae. Issue 1 (1st May 2016)

• Record Type: Journal Article
• Authors: Dever, Thomas E; Kinzy, Terri Goss; Pavitt, Graham D
• Is Part Of: Genetics. Volume 203:Issue 1(2016)
• Page Start: 65
• Page End: 107
• Publication Date: 2016-05-01
• Subjects: GCN4 -- translation elongation -- translation initiation -- YeastBook; Genetics -- Periodicals; 576.5
• DOI: 10.1534/genetics.115.186221
• Languages: English
• ISSNs: 0016-6731

Abstract

Abstract: In this review, we provide an overview of protein synthesis in the yeast Saccharomyces cerevisiae . The mechanism of protein synthesis is well conserved between yeast and other eukaryotes, and molecular genetic studies in budding yeast have provided critical insights into the fundamental process of translation as well as its regulation. The review focuses on the initiation and elongation phases of protein synthesis with descriptions of the roles of translation initiation and elongation factors that assist the ribosome in binding the messenger RNA (mRNA), selecting the start codon, and synthesizing the polypeptide. We also examine mechanisms of translational control highlighting the mRNA cap-binding proteins and the regulation of GCN4 and CPA1 mRNAs.