An MCMC Algorithm for Target Estimation in Real-Time DNA Microarrays. (2nd August 2010)

• Record Type: Journal Article
• Authors: Vikalo Vikalo, Haris Haris; Gokdemir Gokdemir, Mahsuni Mahsuni
• Other Names: Lähdesmäki Lähdesmäki Harri Harri Academic Editor.
• Is Part Of: EURASIP journal on advances in signal processing. Volume 2010(2010)
• Publication Date: 2010-08-02
• Subjects: Signal processing -- Periodicals; Traitement du signal; Signal processing; Periodicals; 621.3822
• DOI: 10.1155/2010/736301
• Languages: English
• ISSNs: 1687-6172

Abstract

Abstract : DNA microarrays detect the presence and quantify the amounts of nucleic acid molecules of interest. They rely on a chemical attraction between the target molecules and their Watson-Crick complements, which serve as biological sensing elements (probes). The attraction between these biomolecules leads to binding, in which probes capture target analytes. Recently developed real-time DNA microarrays are capable of observing kinetics of the binding process. They collect noisy measurements of the amount of captured molecules at discrete points in time. Molecular binding is a random process which, in this paper, is modeled by a stochastic differential equation. The target analyte quantification is posed as a parameter estimation problem, and solved using a Markov Chain Monte Carlo technique. In simulation studies where we test the robustness with respect to the measurement noise, the proposed technique significantly outperforms previously proposed methods. Moreover, the proposed approach is tested and verified on experimental data.