Deep_CNN_LSTM_GO: Protein function prediction from amino-acid sequences. (December 2021)

• Record Type: Journal Article
• Authors: Elhaj-Abdou, Mohamed E.M.; El-Dib, Hassan; El-Helw, Amr; El-Habrouk, Mohamed
• Is Part Of: Computational biology and chemistry. Volume 95(2021)
• Publication Date: 2021-12
• Subjects: Deep learning -- Gene ontology -- CNN -- LSTM -- Protein function prediction -- MF -- BP -- CC -- UniProt-SwissProt -- CAFA; Chemistry -- Data processing -- Periodicals; Biology -- Data processing -- Periodicals; Biochemistry -- Data processing; Biology -- Data processing; Molecular biology -- Data processing; Periodicals; Electronic journals; 542.85
• DOI: 10.1016/j.compbiolchem.2021.107584
• Languages: English
• ISSNs: 1476-9271

Abstract

Abstract: Protein amino acid sequences can be used to determine the functions of the protein. However, determining the function of a single protein requires many resources and a tremendous amount of time. Computational Intelligence methods such as Deep learning have been shown to predict the proteins' functions. This paper proposes a hybrid deep neural network model to predict an unknown protein's functions from sequences. The proposed model is named Deep_CNN_LSTM_GO. Deep_CNN_LSTM_GO is an Integration between Convolutional Neural network (CNN) and Long Short-Term Memory (LSTM) Neural Network to learn features from amino acid sequences and outputs the three different Gene Ontology (GO). The gene ontology represents the protein functions in the three sub-ontologies: Molecular Functions (MF), Biological Process (BP), and Cellular Component (CC). The proposed model has been trained and tested using UniProt-SwissProt's dataset. Another test has been done using Computational Assessment of Function Annotation (CAFA) on the three sub-ontologies. The proposed model outperforms different methods proposed in the field with better performance using three different evaluation metrics (Fmax, Smin, and AUPR) in the three sub-ontologies (MF, BP, CC). Graphical Abstract: ga1