Interventional effect of processing temperature on anti-angiogenesis of Coptis chinensis and screening of active components by UPLC-MS/MS on quail chick chorioallantoic membrane model. (6th April 2023)

• Record Type: Journal Article
• Authors: Wang, Dan; Qi, Miao; Zhao, Hedi; Wu, Haozhong; Chen, Han; Lan, Yanan; Wang, Yanmin; Jiang, Yani; Wang, Jingjuan
• Is Part Of: Journal of ethnopharmacology. Volume 305(2023)
• Publication Date: 2023-04-06
• Subjects: Anti-angiogenesis -- Coptis chinensis -- Processing temperature -- Alkaloids -- Quail chick chorioallantoic membrane; CC Coptis chinensis -- TCM Traditional Chinese medicine -- q-CAM quail chick chorioallantoic membrane -- CAM chick chorioallantoic membrane model -- HUVECs human umbilical vein endothelial cells -- ED embryonic day -- DMSO dimethyl sulfoxide -- VA vascular area -- ESI electrospray ionization -- DE differential expression -- VEGF vascular endothelial growth factor -- ECs endothelial cells; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2022.116014
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Coptis chinensis Franch. (CC), as a commonly used heat-clearing and toxin-resolving traditional Chinese herbal medicine, has gained increased attention for its anti-tumor activity. However, little is known about the anti-tumor angiogenesis effect of CC and its possible bioactive components. Also, it has been shown that temperature affects the quality of CC, albeit whether and how it affects the anti-angiogenic activity of CC is currently unknown. Aim of the study: To determine the processing temperatures (40, 60, 80, 120, 140, 150, 160 and 200 °C) at which CC has the strongest anti-angiogenic effect and speculate the possible bioactive components. Materials and methods: The q-CAM model was constructed to explore the anti-angiogenesis agents of CC. The angiogenesis inhibition effects of CC samples at different processing temperatures and its seven alkaloids were determined based on morphological observation and vascular area proportion analysis. UPLC-MS/MS was employed to screen the potent active components of CC on anti-angiogenesis. Results: All the intervention by CC at different processing temperatures and its seven alkaloids could inhibit angiogenesis on q-CAM vessels, as evidenced by a poor vasular development in morphological observation and a low vascular area proportion in vascular quantitative analysis, most evident in CC processed at 40 °C and palmatine. LC-MS revealed that palmatine displayed strongest inhibitory effect on q-CAM vessels with a high absorption due to its stable structure. And the maternal nucleus transformation phenomenon of CC alkaloids was found in the quail embryo metabolism. Conclusions: The q-CAM models in conjunction with the UPLC-MS/MS technique could be a useful tool for assessing tumor angiogenesis and screening tumor-targeted medicines. Processing temperature can affect the anti-angiogenesis effect of CC because of its function on the content of alkaloids, and palmatine can be considered as a prospective anti-angiogenic drug. Graphical abstract: Image 1 Highlights: New sight on q-CAM models with UPLC-MS/MS for angiogenesis studies. Temperature affect the anti-angiogenesis efficacy of Coptis chinensis. In-depth analysis of alkaloids on vessels; palmatine inhibits angiogenesis better. Performance of the metabolic transformation between protoberberine alkaloids.