IL-23 favours outgrowth of spondyloarthritis-associated pathobionts and suppresses host support for homeostatic microbiota. Issue 4 (30th January 2019)

• Record Type: Journal Article
• Authors: Rehaume, Linda M; Matigian, Nicholas; Mehdi, Ahmed M; Lachner, Nancy; Bowerman, Kate L; Daly, Joshua; Lê Cao, Kim-Anh; Hugenholtz, Philip; Thomas, Ranjeny
• Is Part Of: Annals of the rheumatic diseases. Volume 78:Issue 4(2019)
• Page Start: 494
• Page End: 503
• Publication Date: 2019-01-30
• Subjects: spondyloarthropathy -- IL-23 -- microbiota -- gastrointestinal tract; Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2018-214381
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Objectives: Certain gut bacterial families, including Bacteroidaceae, Porphyromonadaceae and Prevotellaceae, are increased in people suffering from spondyloarthropathy (SpA), a disease group associated with IL23R signalling variants. To understand the relationship between host interleukin (IL)-23 signalling and gut bacterial dysbiosis in SpA, we inhibited IL-23 in dysbiotic ZAP-70-mutant SKG mice that develop IL-23-dependent SpA-like arthritis, psoriasis-like skin inflammation and Crohn's-like ileitis in response to microbial beta 1, 3-glucan (curdlan). Methods: We treated SKG mice weekly with anti-IL-23 or isotype mAb for 3 weeks, rested them for 3 weeks, then administered curdlan or saline. We collected faecal samples longitudinally, assessed arthritis, spondylitis, psoriasis and ileitis histologically, and analysed the microbiota community profiles using next-generation sequencing. We used multivariate sparse partial least squares discriminant analysis to identify operational taxonomic unit (OTU) signatures best classifying treatment groups and linear regression to develop a predictive model of disease severity. Results: IL-23p19 inhibition in naïve SKG mice decreased Bacteroidaceae, Porphyromonadaceae and Prevotellaceae. Abundance of Clostridiaceae and Lachnospiraceae families concomitantly increased, and curdlan-mediated SpA development decreased. Abundance of Enterobacteriaceae and Porphyromonadaceae family and reduction in Lachnospiraceae Dorea genus OTUs early in disease course were associated with disease severity in affected tissues. Conclusions: Dysbiosis in SKG mice reflects human SpA and is IL-23p19 dependent. In genetically susceptible hosts, IL-23p19 favours outgrowth of SpA-associated pathobionts and reduces support for homeostatic-inducing microbiota. The relative abundance of specific pathobionts is associated with disease severity.