A highly purified C-phycocyanin from thermophilic cyanobacterium Thermosynechococcus elongatus and its cytotoxic activity assessment using an in vitro cell-based approach. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: El-Mohsnawy, Eithar; Abu-Khudir, Rasha
• Is Part Of: Journal of Taibah University for science. Volume 14:Issue 1(2020)
• Page Start: 1218
• Page End: 1225
• Publication Date: 2020-01-01
• Subjects: C-phycocyanin -- extraction -- purification -- anti-cancer activity -- cyanobacteria; Science -- Periodicals; Science; Periodicals; 505
• DOI: 10.1080/16583655.2020.1812287
• Languages: English
• ISSNs: 1658-3655

Abstract

ABSTRACT: C-phycocyanin (C-PC) is a protein–pigment widely used in nutrition and pharmaceutical applications. High purity of C-PC is a limiting factor required for its medical use; hence purity improvement with successful plausible pharmaceutical application is the state of the art of the present work. Highly purified C-PC (A620 /A280 = 6.6) was obtained from Thermosynechococcus elongatus . Combined enzymatic cell wall degradation, cellular exposure to high pressure, sonication, and one-time homogenization was found to be an effective purification protocol. Purified complex was confirmed by SDS-PAGE and UV-absorption spectral analysis. Cytotoxic effect of the obtained C-PC was assessed in vitro using a panel of cancer cells. Results showed that MCF-7 cells were most sensitive to C-PC (IC50 = 158.9 µM) compared to Caco-2 (IC50 = 258.3 µM) and HepG2 (IC50 = 277.5 µM) cells. Our findings shed the light on the potential medical use of C-PC isolated from T. elongatus in controlling cancer cells.